Hitachi Software’s MiraiBio Group has recently launched 2 new software tools in the DNASIS SmartNote web application: the Allele Specific Primer Extension (ASPE) Assay Design tool and the xTAG Software (formerly TagIT) tool. These tools are important time savers for those who are interested in multiplexing their SNP or genotyping assays on the Luminex xMAP (also sold by Bio-Rad as the Bio-Plex) platform.
The ASPE Assay Design tool designs probes and primers by optimizing melting temperatures and screening for cross reactivity among the probe and primer sets. All you have to do is to provide the tool with your sequences, SNP position, and allele variants (if you have the SNP rs accession number this information will automatically be populated ). You will get a comprehensive report on the amplification primer pairs that are recommended along with the tagged allele specific primers and the corresponding MicroPlex xTAG Microspheres (formerly FlexMAP beads) to use in your assay. You will even be able to purchase the Microspheres directly from Hitachi Software! It doesn’t get any easier than this.
If you have already designed your allele specific primers, then you have the option of using the xTAG Software tool to select the right MicroPlex xTAG Microspheres to use in your assay.
DNASIS SmartNote is a free web application that is an in silico lab notebook for molecular biologists. Use it to:
Hay varios archivos que no puedo abri, ¿Cómo pueden ayudarme?, gacias Diana
Reply
aliu reply on December 30th, 2008 10:49 am:
Hi Diana,
I apologize for not being able to write directly in Spanish but which files are you referring to? If you are talking about the .csv file that you can download from the ASPE Probe Design tool and the xTAG Software, then this file can be opened in any text editor such as Notepad or even spreadsheet software such as Excel.
I hope this information helps.
Reply
Add A Comment