Comments on: Top 10 Tips for Luminex/Bio-Plex® Data Analysis http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/ What's going on at MiraiBio Fri, 03 Feb 2012 21:35:25 +0000 hourly 1 http://wordpress.org/?v= By: Allen Liu http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/comment-page-1/#comment-44405 Allen Liu Fri, 03 Feb 2012 21:35:25 +0000 http://www.miraibio.com/blog/?p=191#comment-44405 Hi, That is a very good question. It is for the specific bead count of beads that are bound with the target and corresponding detection antibodies. Allen Hi,

That is a very good question. It is for the specific bead count of beads that are bound with the target and corresponding detection antibodies.

Allen

]]> By: Y.Ma http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/comment-page-1/#comment-44396 Y.Ma Fri, 03 Feb 2012 20:21:30 +0000 http://www.miraibio.com/blog/?p=191#comment-44396 Is the bead count only counting the beads that are bound with the target and the detection antibodies? Or is it the total bead count in the well that has the bound and the unbound beads? Thanks. Is the bead count only counting the beads that are bound with the target and the detection antibodies? Or is it the total bead count in the well that has the bound and the unbound beads?

Thanks.

]]> By: aliu http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/comment-page-1/#comment-6205 aliu Wed, 28 Oct 2009 17:00:09 +0000 http://www.miraibio.com/blog/?p=191#comment-6205 Hi Min Shi, The 35 minimum bead count was something that everyone is told during the Luminex trainings but we never received any supporting documentation on it. I recently talked with a Luminex tech support person who did a lot of digging and told me that Luminex did in fact hire some statisticians to do the study around 1999 and they came up with that number. The reason why this study was never published was because the instrumentation was changed. I was told that the changes were not really significant but, to play it safe, Luminex decided not to publish. I hope this helps. Hi Min Shi,

The 35 minimum bead count was something that everyone is told during the Luminex trainings but we never received any supporting documentation on it. I recently talked with a Luminex tech support person who did a lot of digging and told me that Luminex did in fact hire some statisticians to do the study around 1999 and they came up with that number. The reason why this study was never published was because the instrumentation was changed. I was told that the changes were not really significant but, to play it safe, Luminex decided not to publish.

I hope this helps.

]]> By: Min Shi http://www.miraibio.com/blog/2009/02/tips-for-luminex-data-analysis/comment-page-1/#comment-6194 Min Shi Mon, 26 Oct 2009 20:47:46 +0000 http://www.miraibio.com/blog/?p=191#comment-6194 Hi, do you know any literature/publication that may support the idea that "35 is the minimum bead count needed for statistically significant analyses"? We sometimes run into "insufficent beads count" issues. It would be great if we can justfy that the samples with 35-100 beads count are still reliable. Hi, do you know any literature/publication that may support the idea that “35 is the minimum bead count needed for statistically significant analyses”? We sometimes run into “insufficent beads count” issues. It would be great if we can justfy that the samples with 35-100 beads count are still reliable.

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