The Hitachi Software MiraiBio group has just released a new build for MasterPlex ReaderFit, the quantitative analysis and curve-fitting data analysis software for microplate readers. New features and improvements include:

• File Import Wizard – Although MasterPlex ReaderFit can automatically import some of the known file formats from common plate readers that are out there in the market, it does not handle all 100% of them. Unknown file formats can now be handled by the File Import Wizard in 1 of 2 ways:
  1. Plate Format

     

  2. List

     

  More details on using the File Import Wizard for MasterPlex ReaderFit

• Best Fit Options – The Best Fit feature now offers 3 parameters for which you can optimize for to determine the best curve fit:
  1. Root Mean Square Error (RMSE) – RMSE is a good measure of accuracy or goodness of fit which aggregates the individual differences or residuals between your expected and calculated values into a single measure of predictive power. This is the default option as it is the one that is recommended.
  2. Standard Deviation of % Recovery – This option will provide the model equation and weighting algorithm combination with the lowest standard deviation of percent recovery. The percent recovery is basically the calculated concentration – the expected concentration * 100.
  3. R-squared – This option is the only Best Fit option in previous builds of MasterPlex ReaderFit. It is not as sensitive to the goodness of fit as the RMSE option.

  

• Competitive Binding and Inhibition Assays – This build provides improved support for competitive binding or inhibition assays.

  

• More Intuitive Auto Fill – The Auto Fill feature helps to automate the entry of the standard concentration values for serial dilutions. This feature has been simplified with more intuitive dilution direction buttons and being able to preview the standard values.

  

Try it out and download the free trial now!