The following webinar recordings have been made available for viewing at your own leisure:
- Reduce cost and length by 50% or more on whole-genome sequencing projects
View Webinar | PowerPoint Presentation - ELISA Curve-Fitting Analysis with ReaderFit.com
View Webinar - How can Optical Mapping accelerate my research?
View Webinar | PowerPoint Presentation - ELISA Curve-Fitting Analysis with ReaderFit Desktop
View Webinar - Quantitative Multiplex Data Analysis with MasterPlex QT
View Webinar - MasterPlex: One Quantitative Analysis Software Solution for Multiple Instruments
PowerPoint Presentation - Getting the Most Out of Your Multiplex Data
PowerPoint Presentation
Pingback: Dose Response Curves with EC50 & IC50 Determination using MasterPlex ReaderFit
Dear Sir/Madam,
My name is Nadia. I was watching your webinar, titled “How to improve your results with Masterplex QT” with Allen Liu. I have a question about weighting. If the weighting does not improve my data’s % recovery; can I choose not to use weighting and still have solid data?
Thank you.
Looking forward hearing from you.
Sincerely,
Nadia Deashinta
University of Nevada, Las Vegas
Dear Nadia,
I would incorporate weighting regardless because it will still affect the calculation of the parameters. The 4PL and 5PL assumes that you have homoscedastic data which, of course, is untrue.
Please let me know if you have any other questions.
Best Regards,
Allen Liu
Dear Mr. Liu,
Another quick question. If for example we have duplicates on standard wells, one of the wells has %recovery of 142 (above 130; not quite acceptable from my understanding) and the other one has %recovery of 84. Is this particular standard is usable (in terms of their mean %recovery is 113)?
Thank you.
Sincerely
Nadia Deashinta
Hi Nadia,
I have seen many different protocols so I really cannot say that what is right or what is wrong. I commonly see protocols where only points that are within 70-130% recovery are accepted and any replicates outside of this range are marked as outliers.
Best Regards,
Allen Liu
I want to know that in one paper of biorad tech note ( u recommended to some one ) i read i done understand that in one table page no 3 hoe they calculated observed value ,please reply
Hi Prerna,
Can you direct me to the Biorad artical that you are referring to? I’m not quite sure what you mean.
Thank you,
Charles Ma