Comments on: View MiraiBio Webinar Series Recordings http://www.miraibio.com/blog/2009/09/view-masterplex-webinar-series-recordings/ What's going on at MiraiBio Fri, 03 Feb 2012 21:35:25 +0000 hourly 1 http://wordpress.org/?v= By: aliu http://www.miraibio.com/blog/2009/09/view-masterplex-webinar-series-recordings/comment-page-1/#comment-9344 aliu Tue, 12 Oct 2010 00:43:49 +0000 http://www.miraibio.com/blog/?p=397#comment-9344 Hi Nadia, I have seen many different protocols so I really cannot say that what is right or what is wrong. I commonly see protocols where only points that are within 70-130% recovery are accepted and any replicates outside of this range are marked as outliers. Best Regards, Allen Liu Hi Nadia,

I have seen many different protocols so I really cannot say that what is right or what is wrong. I commonly see protocols where only points that are within 70-130% recovery are accepted and any replicates outside of this range are marked as outliers.

Best Regards,

Allen Liu

]]> By: Nadia Deashinta http://www.miraibio.com/blog/2009/09/view-masterplex-webinar-series-recordings/comment-page-1/#comment-9343 Nadia Deashinta Mon, 11 Oct 2010 21:09:28 +0000 http://www.miraibio.com/blog/?p=397#comment-9343 Dear Mr. Liu, Another quick question. If for example we have duplicates on standard wells, one of the wells has %recovery of 142 (above 130; not quite acceptable from my understanding) and the other one has %recovery of 84. Is this particular standard is usable (in terms of their mean %recovery is 113)? Thank you. Sincerely Nadia Deashinta Dear Mr. Liu,

Another quick question. If for example we have duplicates on standard wells, one of the wells has %recovery of 142 (above 130; not quite acceptable from my understanding) and the other one has %recovery of 84. Is this particular standard is usable (in terms of their mean %recovery is 113)?

Thank you.

Sincerely
Nadia Deashinta

]]> By: aliu http://www.miraibio.com/blog/2009/09/view-masterplex-webinar-series-recordings/comment-page-1/#comment-9342 aliu Mon, 11 Oct 2010 20:52:50 +0000 http://www.miraibio.com/blog/?p=397#comment-9342 Dear Nadia, I would incorporate weighting regardless because it will still affect the calculation of the parameters. The 4PL and 5PL assumes that you have homoscedastic data which, of course, is untrue. Please let me know if you have any other questions. Best Regards, Allen Liu Dear Nadia,

I would incorporate weighting regardless because it will still affect the calculation of the parameters. The 4PL and 5PL assumes that you have homoscedastic data which, of course, is untrue.

Please let me know if you have any other questions.

Best Regards,

Allen Liu

]]> By: Nadia Deashinta http://www.miraibio.com/blog/2009/09/view-masterplex-webinar-series-recordings/comment-page-1/#comment-9340 Nadia Deashinta Mon, 11 Oct 2010 17:56:58 +0000 http://www.miraibio.com/blog/?p=397#comment-9340 Dear Sir/Madam, My name is Nadia. I was watching your webinar, titled "How to improve your results with Masterplex QT" with Allen Liu. I have a question about weighting. If the weighting does not improve my data's % recovery; can I choose not to use weighting and still have solid data? Thank you. Looking forward hearing from you. Sincerely, Nadia Deashinta University of Nevada, Las Vegas Dear Sir/Madam,

My name is Nadia. I was watching your webinar, titled “How to improve your results with Masterplex QT” with Allen Liu. I have a question about weighting. If the weighting does not improve my data’s % recovery; can I choose not to use weighting and still have solid data?

Thank you.
Looking forward hearing from you.

Sincerely,
Nadia Deashinta
University of Nevada, Las Vegas

]]> By: Dose Response Curves with EC50 & IC50 Determination using MasterPlex ReaderFit http://www.miraibio.com/blog/2009/09/view-masterplex-webinar-series-recordings/comment-page-1/#comment-8038 Dose Response Curves with EC50 & IC50 Determination using MasterPlex ReaderFit Mon, 15 Mar 2010 16:17:27 +0000 http://www.miraibio.com/blog/?p=397#comment-8038 [...] Webinars [...] [...] Webinars [...]

]]>