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Posted by Allen Liu under MasterPlex QT, MasterPlex ReaderFit

The 5-PL or 5 Parameter Logistic is a nonlinear regression model used for prediction of the probability of occurrence of an event by fitting data to a logistic curve. It differs from the 4-PL or 4 Parameter Logistic model in that it is an asymmetric function which is a better fit for immunoassay or bioassay data.  As the name suggests, there are 5 parameters in the 5-PL model equation:

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