The importance of weighting with the 4PL and 5PL
We recently had a customer that was interested in MasterPlex QT because his current analysis software for his Bio-Plex instrument was reporting a lot of “OOR < ” or out of range concentration values (below the lower asymptote in this case) for points on the lower end of curve. This is what you would normally expect to see for values that fall below the minimum asymptote BUT the software did not have the capability to use weighting in calculating the lower asymptote which can greatly affect points on the lower part of the curve.
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