A new free update for MasterPlex 2010 has just been released with some powerful new features that make it that much easier to do curve fitting with MasterPlex. If you are a current customer, please download the installer (QT download link, ReaderfFit download link, EX download link) and launch it to do the upgrade. Here is the list of new features and changes by module:

MasterPlex ReaderFit

• Improve 0 Standard Handling – If the user indicates a zero standard, MasterPlex will automatically use it to define the limits of the curve. This is especially useful in defining the upper limits of the curve for competitive binding assays.
• New Calculation on Control Wells – When Control Groups are indicated as the sample type, users can now enter expected values for them and see the residual and % recovery calculations for the control samples.
  
  
• Failed QC Column in View Results tab – The data table in the View Results tab now has a new data field called QC Flags which display why samples have failed the Quality Control Manager.
  
• Templates for Quality Control Manager – The MasterPlex templating system is available for the QC Manager to allow users to quickly load up previously used QC thresholds for quick time-savings and consistent analyses.
• Glide Feature for Curve – Glide along the curve and get instant x and y values for any point on the curve.
  
• Real-time Status Updates on Calculations – To further improve data integrity, there are status updates now to remind users that they have made changes that affect the calculated values (i.e. edited dilution factors, marked outliers) and that a re-calculation is required.
  
• LLOD and ULOD Values Now Displayed – The LLOD and ULOD values in the Quality Control Manager are now displayed

MasterPlex QT

• Improved Support for Meso Scale Discovery Compatibility – There is improved support for the MSD platform export data file.
• Improve 0 Standard Handling – If the user indicates a zero standard, MasterPlex will automatically use it to define the limits of the curve. This is especially useful in defining the upper limits of the curve for competitive binding assays.
• New Calculation on Control Wells – When Control Groups are indicated as the sample type, users can now enter expected values for them and see the residual and % recovery calculations for the control samples.
  
  
• Failed QC Column in View Results tab – The data table in the View Results tab now has a new data field called QC Flags which display why samples have failed the Quality Control Manager.
  
• Templates for Quality Control Manager – The MasterPlex templating system is available for the QC Manager to allow users to quickly load up previously used QC thresholds for quick time-savings and consistent analyses.
• Glide Feature for Curve – Glide along the curve and get instant x and y values for any point on the curve.
  
• Real-time Status Updates on Calculations – To further improve data integrity, there are status updates now to remind users that they have made changes that affect the calculated values (i.e. edited dilution factors, marked outliers) and that a re-calculation is required.
  
• LLOD and ULOD Values Now Displayed – The LLOD and ULOD values in the Quality Control Manager are now displayed

MasterPlex EX

• Improved Support for Meso Scale Discovery Compatibility – There is improved support for the MSD platform export data file.
• Failed QC Column in View Results tab – The data table in the View Results tab now has a new data field called QC Flags which display why samples have failed the Quality Control Manager.
  
• Templates for Quality Control Manager – The MasterPlex templating system is available for the QC Manager to allow users to quickly load up previously used QC thresholds for quick time-savings and consistent analyses.
• Real-time Status Updates on Calculations – To further improve data integrity, there are status updates now to remind users that they have made changes that affect the calculated values (i.e. edited dilution factors, marked outliers) and that a re-calculation is required.
  
• LLOD and ULOD Values Now Displayed – The LLOD and ULOD values in the Quality Control Manager are now displayed

The Hitachi Software MiraiBio group has just released a new build for MasterPlex ReaderFit, the quantitative analysis and curve-fitting data analysis software for microplate readers. New features and improvements include:

• File Import Wizard – Although MasterPlex ReaderFit can automatically import some of the known file formats from common plate readers that are out there in the market, it does not handle all 100% of them. Unknown file formats can now be handled by the File Import Wizard in 1 of 2 ways:
  1. Plate Format
  2. List

  More details on using the File Import Wizard for MasterPlex ReaderFit

• Best Fit Options – The Best Fit feature now offers 3 parameters for which you can optimize for to determine the best curve fit:
  1. Root Mean Square Error (RMSE) – RMSE is a good measure of accuracy or goodness of fit which aggregates the individual differences or residuals between your expected and calculated values into a single measure of predictive power. This is the default option as it is the one that is recommended.
  2. Standard Deviation of % Recovery – This option will provide the model equation and weighting algorithm combination with the lowest standard deviation of percent recovery. The percent recovery is basically the calculated concentration – the expected concentration * 100.
  3. R-squared – This option is the only Best Fit option in previous builds of MasterPlex ReaderFit. It is not as sensitive to the goodness of fit as the RMSE option.

  

• Competitive Binding and Inhibition Assays – This build provides improved support for competitive binding or inhibition assays.
• More Intuitive Auto Fill – The Auto Fill feature helps to automate the entry of the standard concentration values for serial dilutions. This feature has been simplified with more intuitive dilution direction buttons and being able to preview the standard values.

MiraiBio offers 2 powerful curve-fitting quantitative analysis solutions that utilizes our time-tested 5-PL nonlinear regression model as well as many others:

ReaderFit Desktop – Robust curve-fitting, quality control and reporting desktop software

ReaderFit.com – Free online curve-fitting application

The Hitachi Software MiraiBio Group has just released a new build of MasterPlex QT v4.0 that boasts several new improvements:

• Best Fit Options – The Best Fit feature now offers 3 parameters for which you can optimize for to determine the best curve fit:
  1. Root Mean Square Error (RMSE) – RMSE is a good measure of accuracy or goodness of fit which aggregates the individual differences or residuals between your expected and calculated values into a single measure of predictive power. This is the default option as it is the one that is recommended.
  2. Standard Deviation of % Recovery – This option will provide the model equation and weighting algorithm combination with the lowest standard deviation of percent recovery. The percent recovery is basically the calculated concentration – the expected concentration * 100.
  3. R-squared – This option is the only Best Fit option in previous builds of MasterPlex QT v4.0. It is not as sensitive to the goodness of fit as the RMSE option.

  

• Competitive Binding and Inhibition Assays – This build provides improved support for competitive binding or inhibition assays.
• More Intuitive Auto Fill – Auto Fill is simplified with more intuitive dilution direction buttons and being able to preview the standard values.

Try MasterPlex QT Desktop – The World’s First Multiplex Curve-Fitting Software for the Luminex 100 and Luminex 200 xMAP Platforms