The Hitachi Software MiraiBio Group Blog

DNASIS MAX has the ability to search for homologous sequences to your sequence with BLAST (Basic Local Alignment Search Tool). BLAST finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

The following procedure guides you through utilizing BLAST against NCBI’s sequence databases.

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DNASIS MAX has the ability to search for homologous sequences to your sequence with Blast which is
useful in judging if the sequence is novel or even add annotations from known gene information. The following procedure guides you through utilizing Blast against an internal sequence database.

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DNASIS MAX uses the Primer3 engine for designing primers, which is one of the most frequently used procedures in daily research.  The following procedure guides you through designing primers utilizing the DNASIS MAX interface.

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New this week:

1. By popular demand, you can now import GenBank and multi-GenBank files into SmartNote, including those exported from other programs, such as DNASIS Max and Vector NTI.

2. We have added a new option for displaying the predicted amino acid sequence aligned with a DNA sequence. Use the ExPASy Translate tool and specify “Includes Nucleotide Sequence” for the “Output Format” option. Thank you, Jessica Min for this suggestion. This should help users who need to design primers for mutagenesis.

3. Support for IUPAC codes in sequences is also being added this week.

Please keep the feedback coming!