Hitachi Solutions America, Ltd. partners with ELISAlink.com

Posted on September 28, 2011 by Robert Lynde

The MiraiBio group of Hitachi Solutions America, Ltd. (HISAL) has partnered with ELISAlink.com to offer ReaderFit.com, a free online curve fitting tool for ELISA analysis, to ELISAlink.com users.

ELISALink.com is an online ELISA community, knowledge base and e-commerce site focused on serving the global ELISA Industry. The site will be anchored by a community-generated ELISA knowledge base and discussion platform and also feature direct access to a wide range of ELISA kits, components, equipment and services.

ReaderFit.com is a free online curve fitting application that allows users to both fit curves and optionally interpolate unknown values off the curve. Users upload response and independent values and then choose from one of 6 model equations: 4PL, 5PL, Quadratic, Log-Logit, Log-Log or Linear and one of four optional weighting algorithms: 1/Y, 1/Y2, 1/X and 1/X2. The resulting curve image and calculated results can then be easily exported from the web application.

“ELISAlink.com is an exciting concept that leverages “web 2.0” and social media features in a way that has been missing in the life science community. People in general have come to expect and rely on some of things that ELISAlink.com offers in other parts of their life. ELISA researchers now have a place to go for all of their ELISA needs. We are excited to offer ReaderFit.com as added value at ELISAlink.com,” said Robert Lynde, Deputy Director of the MiraiBio Group of HISAL.

“The team at HISAL really got it right with ReaderFit. The program is intuitive and designed from the perspective of an ELISA user, providing a streamlined and easy to use platform that is an invaluable tool to researchers and clinical users alike. The powerful Desktop Edition and the 21 CFR Part 11 compliant Security Edition were complimented earlier this year with a completely Online Edition – a natural fit for ELISAlink.com and our mission to provide the best and most powerful ELISA resources on the web,” said David Barka, Founder of ELISAlink.com

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Microplate Reader Data Analysis Does Not Get Any Easier

Posted on June 4, 2009 by Charles Ma

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Quick Demo of MasterPlex ReaderFit (1:43 min.)

The microplate analysis demo includes the following simple steps:

Importing an Excel file with the RLU values
Marking the background, standard, and unknown samples
Filling in the standard concentrations with the AutoFill Feature
Pressing the Best Fit button
Reviewing standard curve, concentrations, and charts

Save time, stop copying & pasting, and download the FREE MasterPlex ReaderFit trial and see for yourself how easy it can be!

ReaderFit Desktop – Robust curve-fitting, quality control and reporting desktop software
Download Free Trial

New File Import Wizard and Best Fit options for MasterPlex ReaderFit

Posted on June 2, 2009 by Charles Ma

The Hitachi Software MiraiBio group has just released a new build for MasterPlex ReaderFit, the quantitative analysis and curve-fitting data analysis software for microplate readers. New features and improvements include:

File Import Wizard – Although MasterPlex ReaderFit can automatically import some of the known file formats from common plate readers that are out there in the market, it does not handle all 100% of them. Unknown file formats can now be handled by the File Import Wizard in 1 of 2 ways:
1. Plate Format
2. List
More details on using the File Import Wizard for MasterPlex ReaderFit
Best Fit Options – The Best Fit feature now offers 3 parameters for which you can optimize for to determine the best curve fit:
1. Root Mean Square Error (RMSE) – RMSE is a good measure of accuracy or goodness of fit which aggregates the individual differences or residuals between your expected and calculated values into a single measure of predictive power. This is the default option as it is the one that is recommended.
2. Standard Deviation of % Recovery – This option will provide the model equation and weighting algorithm combination with the lowest standard deviation of percent recovery. The percent recovery is basically the calculated concentration – the expected concentration * 100.
3. R-squared – This option is the only Best Fit option in previous builds of MasterPlex ReaderFit. It is not as sensitive to the goodness of fit as the RMSE option.
Competitive Binding and Inhibition Assays – This build provides improved support for competitive binding or inhibition assays.
More Intuitive Auto Fill – The Auto Fill feature helps to automate the entry of the standard concentration values for serial dilutions. This feature has been simplified with more intuitive dilution direction buttons and being able to preview the standard values.

MiraiBio offers 2 powerful curve-fitting quantitative analysis solutions that utilizes our time-tested 5-PL nonlinear regression model as well as many others:

ReaderFit Desktop – Robust curve-fitting, quality control and reporting desktop software

ReaderFit.com – Free online curve-fitting application