Hitachi Software recently released a free software tool to automate the design of SNP genotyping assays using Allele Specific Primer Extension (ASPE) on the xMAP platform. The ASPE approach utilizes Luminex Corporation’s xTAG (formerly FlexMAP) microspheres which are pre-coupled to oligonucleotide sequences (anti-tags) that are optimized to be an isothermal set and to have minimum cross-reactivity. The detection probes have the tag sequence synthesized on the 5′ end. The use of the pre-coupled xTAG microspheres simplifies the assay greatly. Read the rest of this entry »
Posted by rlynde under Microspheres, SmartNote
Posted by aliu under SmartNote
Hitachi Software’s MiraiBio Group has recently launched 2 new software tools in the DNASIS SmartNote web application: the Allele Specific Primer Extension (ASPE) Assay Design tool and the xTAG Software (formerly TagIT) tool. These tools are important time savers for those who are interested in multiplexing their SNP or genotyping assays on the Luminex xMAP (also sold by Bio-Rad as the Bio-Plex) platform.


