Loading ...Many of the FlexMAP (now called xTAG) microsphere users relied on the TagIT software to help choose which microsphere sets to use in genetic detection assays run on the xMAP platform. When the TagIT software was removed these users no longer had a reliable way to design their assays. Well the good news is that TagIT is back!
Hitachi Software recently released a free software tool to automate the design of SNP genotyping assays using Allele Specific Primer Extension (ASPE) on the xMAP platform. The ASPE approach utilizes Luminex Corporation’s xTAG (formerly FlexMAP) microspheres which are pre-coupled to oligonucleotide sequences (anti-tags) that are optimized to be an isothermal set and to have minimum cross-reactivity. The detection probes have the tag sequence synthesized on the 5′ end. The use of the pre-coupled xTAG microspheres simplifies the assay greatly.
