Software dedicated to Elisa Data Analysis.
Get Fast Accurate Results Like Never Before.
Curve-fitting for Elisa analysis has never been easier with full support for the 4 Parameter Logistic (4-PL) and 5 Parameter Logistic (5-PL) model equations and much more!
Get Fast Accurate Results Like Never Before.
How will MasterPlex ReaderFit benefit you?
- Save Time
- Best Fit
Let the software do the work. This feature automatically selects the optimal model equation and weighting algorithm with different parameters to optimize for: Root Mean Square Error (RMSE), R-Square, and Standard Deviation of % Recovery.
- Templates
Find yourself doing the same assay over and over again? MasterPlex ReaderFit can save your plate layout and preferences into templates. The next time you have a similar data file, just import the template and the analysis will be done for you in seconds. - Merge Plates
The Virtual Plate feature allows you to expand beyond the instrument or assay limitations and combine data from 2 or more result files:- Quantitate samples on one result set using standards from another result set
- Monitor standards over time
- Best Fit
- Automatic Outlier Detection
- Automatically flag outliers based on:
- Raw Value
- Concentration
- Quality Control Manager Add-on (Optional): New!
- Lower Limit of Detection (LLOD) - Automatically calculate your LLOD on a per analyte basis from the mean of the selected background wells plus the number of standard deviations you want defined
- Percent Coefficient of Variation (% CV) - Define your maximum % CV threshold
- Extrapolated Values - Automatically exclude extrapolated data from your results
- Automatically flag outliers based on:
- Reliability
- Optimized Model Equations
Our time-tested model equations offer accurate and precise curve-fitting from one experiment to the next. - Weighting Algorithms
The nature of the data carries with it a variance of the dependent variable that changes across the data which is known as heteroscedasticity. Many methods in regression analysis are based on an assumption of equal variance but MasterPlex ReaderFit offers 4 different weighting algorithms to account for heteroscedasticity.
- Optimized Model Equations
- Reporting
- Spreadsheet style pivot tables allow for easy viewing and manipulation of your results.
- Highly Flexible Graphing Capabilities
Create customized publication quality charts and graphs with a few clicks.
- Custom Report Generator
The spreadsheet style pivot tables does not generate the report you want? This is where our Custom Report Generator feature comes in. Using custom report templates which we can design for you, any report format is possible whether you need it in Excel, XML, HTML, or just plain text. This is especially useful if you have a LIMS and require a certain XML formatted input file.
- Spreadsheet style pivot tables allow for easy viewing and manipulation of your results.
- Save Money
- Share Licenses
Networking licensing helps you save money by allowing you to install MasterPlex QT on as many computers as you need while only paying for the software that you actually use. - Cut down the amount of time spent on analysis with powerful time-saving features such as the Best Fit, Templating, and Virtual Plate features.
- Share Licenses
Who We Are
Hitachi Software Engineering Co., Ltd. is a part of the Hitachi family of companies which employs over 400,000 people globally and generates more than $100 billion in annual revenue. Founded in 1910, our company’s vision is to contribute to the solution of fundamental global issues, and pursue the realization of a better, more prosperous global society, in line with Hitachi's founding spirit, utilizing the group's knowledge and technology.
Testimonials
I evaluated several programs before deciding on the MasterPlex ReaderFit software for my ELISAs. Only the MasterPlex ReaderFit software gave me the flexibility I needed and universal format of Excel file import and export to use from results from multiple plate readers. Having a fully functional demo software was also invaluable in my decision making. Probably most important was the level of customer service from MiraiBio. The programmers and engineers even solved my problem of having to do a 4 parameter curve fit on an inhibition ELISA.
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S. Connery
Texas Tech University Health Sciences Center
Texas Tech University Health Sciences Center
We chose MiraiBio's MasterPlex software because our Luminex instrument is shared by several labs, so the software had to be as intuitive as possible to accommodate users with different levels of specific training. It has met and exceeded our expectations in this regard.
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Dr. Mark Scott
University of California, San Francisco
University of California, San Francisco




Watch MasterPlex ReaderFit in Action